Team’s title: RNA Biogenesis and trafficking
Institute: Institute of Molecular Genetics of Montpellier – IGMM
Phone: 00 33 4 34 35 96 47
Our group has strong interests in gene expression mechanisms, from transcription to translation. While we are interested in the regulation of these processes and their functional consequences, the big question that moves us is to understand how they occur in the context of a living cell.
Indeed, cells are not only the individual units where gene regulation takes place, they are also incredible objects: if we consider RNA and proteins, a typical cell contains several hundreds of thousands of different molecular species, with some present in millions of copies per cell while others in only few. In order to function with such a high molecular complexity, cells have evolved a fascinating spatial organization, which is not only pushed to an amazing degree, but is also remarkably plastic and with a high molecular mobility. It is to get insights into these very fundamental questions that we first developed tools to image single mRNAs in live cells (Bertrand et al., 1998; Fusco et al., 2003). With these tools in hands and others that we developed later on (for instance see Pichon et al, 2016; Tantale et al. 2016), we aim at imaging the basic mechanisms of gene expression directly in living cells. We hope to provide a renewed vision of these fundamental processes.
Our strategy is to invest in technological developments to access and image new facets of gene expression. Over the years, we have been able to contribute to the resolution of important issues in the area of gene expression, including transcription, splicing and translation. We currently have four research themes: (i) imaging transcription to characterize the origins and consequences of transcriptional noise; (ii) spatio-temporal regulation of RNA localization and translation; (iii) assembly of macromolecular complexes by the HSP90/R2TP chaperone; (iv) computational identification of non-coding genomic elements.
Keywords: RNA biology, single molecule imaging, transcription, translation, RNP assembly
A localization screen reveals translation factories and widespread co-translational protein targeting. Preprint available at https://ssrn.com/abstract=3300043 Chouaib, R., Safieddine, A., Pichon, X., Kwon, OS., Samacoits, A., Traboulsi, AM., Tsanov, N., Robert, MC., Poser, I., Zimmer, C., Hyman, A. A., Le Hir, H., Zibara, K., Peter, M., Mueller, F.*, and Walter, T.*, Bertrand, E.*
* co-corresponding authors.
A computational framework to study sub-cellular mRNA localization. Nat Comm, 2018, 9:4584. Samacoits, A., Chouaib, R., Safieddine, S., Traboulsi, A., Ouyang, W., Zimmer, C., Peter, M., Bertrand, E.*, Walter, T.*, Mueller, F.*
* co-corresponding authors.
A growing toolbox to image gene expression in single cells: sensitive approaches for demanding challenges. Mol. Cell, 2018, 71:468-480. Pichon, X., Lagha, M., Mueller, F. and Bertrand, E.
Invited review for the 20th anniversary of the journal; featured article of the issue
The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones. Nat Comm, 2018, 9:2093. Maurizy, C., Quinternet, M., Abel, Y., Verheggen, C., Santo, P. E., Bourguet, M., Paiva, A. C. F., Bragantini, B., Chagot, ME., Robert, MC., Abeza, C. Fabra, P., Fort, P., Vandermoere, F., Sousa, P., Rain, JC., Charpentier, B., Cianférani, S., Bandeiras, T. M., Pradet-Balade, B., Manival, X., Bertrand, E.
Comment in Nature Comm, 2018.
The PAQosome, an R2TP-based chaperone for quaternary structure formation. TIBS 2018, 43:4-9. Houry, W.A.*, Bertrand, E.*, and Coulombe, B.*
Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones. J. Cell Biol., 2017, 21:1579-1596. Malinová, A., Cvačková, Z., Matějů, D., Hořejší, Z., Abéza, C., Vandermoere, F., Bertrand, E.*, Staněk, D.*, Verheggen, C.*
Mutually exclusive CBC-containing complexes contribute to RNA fate. Cell Reports, 2017, 18:2635-2650. Giacometti, S., Benbahouche, N. H., Domanski, M., Robert, M-C., Meola, N., Lubas, M., Bukenborg, J., Andersen, J. S., Schultze, W. M., Verheggen, C., Kudla, G.*, Jensen, T. H.*, Bertrand, E.*
Visualization of single polysomes reveals translation dynamics of endogenous mRNAs in living human cells, J. Cell Biol., 2016, 214:769-81. Pichon X., Bastide A., Safieddine A., Chouaib R., Samacoits A., Basyuk E., Peter M., Mueller F., Bertrand E.
Spotlight article, see commentary in doi:10.1083/jcb.201608075, selected by F1000
SmiFISH and FISH-quant – a flexible single mRNA detection approach with super-resolution capability. Nucleic Acids Res, 2016, pii:gwk784, Tsanov, N., Samacoits, A., Chouaib, R., Traboulsi, A.M., Gostan, T., Weber, C., Zimmer, C., Zibara, K., Walter, T., Peter, M.*, Bertrand, E.*, Mueller, F*
A real-time, single molecule view of transcription reveals convoys of RNA polymerases and multiscale bursting. Nat. Comm., 2016, 7:12248. Tantale, K., Müller, F., Kozulic-Pirher, A., Lesne, A., Victor, JL., Robert, MC., Capozi, S., Bäcker, V., Mateos-Langerak, J., Darzacq, X., Zimmer, C., Basyuk, E., Bertrand, E.
Selected by F1000